Microbiome DNA RNA Extraction Kit PCR Metagenomic Next Generation Sequencing

Microbiome cfDNA Isolation Kit (PCR) for Metagenomic Next Generation Sequencing

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product Information

Product Manifestation

Plasma microbiome cell-free DNA (mcfDNA) is a potential biomarker for the diagnosis of the etiology of infection. In the absence of samples from infection foci, the detection of plasma mcfDNA brings important value for pathogen identification. Microbiome cfDNA Isolation Kit (PCR) for Metagenomic Next Generation Sequencing uses magnetic beads with a unique separation function and a unique buffer system to rapidly extract mcfDNA from fresh or frozen plasma, easily achieving low concentration of trace nucleic acid extraction.

Methodology

Macro-genome next-generation sequencing (mNGS):the entire microbial composition of the collected samples was analyzed using high-throughput sequencing technology.

Product Advantages

1. High Quality: The extraction process does not require Carrier RNA, effectively removes impurities/ downstream PCR inhibition factors and facilitates accurate quantification.
2. Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.
3. Low Microbial Contamination: Effectively control contamination by exogenous pathogenic micro-organisms.
4. Broad Compatibility: Compatible with manual and automatic extraction programs, adapted to Tecan, Hamilton, PE and other nucleic acid extraction workstations.

Company Advantages

1. Production:Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard
2. Application:Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators

Figure 1-2. Plasma mcfDNA extraction and library preparation with high consistency with competitive products

mcfDNA was extracted from plasma samples using the Microbiome cfDNA Isolation Kit (PCR) for Metagenomic Next Generation Sequencing and Qiagen's comparable products, and library preparation was performed at equal quality. The extraction yield and library preparation yield of BunnyMag kits were consistent with those of competing products.

Main Composition

Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage Condition

Please take out the magnetic beads and proteinase K and store at 2-8℃; others store at (10-30℃), the validity period is 12 months.

Operating Process