Body Fluids Cell Free DNA Isolation Kit Sanger Sequencing Genetic Testing

Body Fluids Cell-free DNA Isolation Kit (ddPCR, mNGS Library Preparation, Sanger Sequencing) for Genetic Testing

Cell-free DNA (cfDNA) is a fragment of cfDNA released from cells into the bloodstream that can carry biological information from tumors, pathogens and fetuses. The analysis of cfDNA provides key markers for diagnosis, detection and monitoring of disease progression. Currently, this technology has been widely used in the fields of tumor mutation detection, targeted drug guidance and prognosis, pathogen detection and non-invasive prenatal screening. Body Fluids Cell-free DNA Isolation Kit (ddPCR, mNGS Library Preparation, Sanger Sequencing) for Genetic Testing uses magnetic beads with a unique separation function and a unique buffer system to extract high quality cfDNA from 0.5-4 mL of fresh or frozen plasma, serum, pleural fluid, ascites, cerebrospinal fluid and other cell-free body fluids.

Advantages

1. Technique:Super paramagnetic beads, which are rapidly aggregated in the magnetic field; uniform size to ensure sufficient magnetic responsiveness without settling; buffer optimized by multiple rounds of proportioning
2. Production:Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard
3. Application:Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators

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Figure 1. Detected mutation abundance is in consistent with the theoretical abundance of the reference standard with a ratio of 1

After using Body Fluids Cell-free DNA Isolation Kit (ddPCR, mNGS Library Preparation, Sanger Sequencing) for Genetic Testing to extract standard cfDNA into NGS process, the detected mutation abundance is highly consistent with the theoretical mutation abundance of ref. standard, and the similarity of mutation abundance detection with similar products of competing companies is high.

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Please take out the magnetic beads and proteinase K and store at 2-8℃; others store at (10-30℃), the validity period is 12 months.